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Wei Sheng Yan Jiu. 2012 Nov;41(6):963-5, 970.

[Determination of kinds of monoamine neurotransmitters in Alzheimer's blood plasma by high performance liquid chromatography].

[Article in Chinese]

Author information

  • 1College of Public Health, Harbin Medical University, Harbin 150081, China.



A method detecting norepinephrine, epinephrine, dopamineand serotonin in Alzheimer's blood plasma by high performance liquid chromatography was developed.


C18 column was used, the mobile phase was composed of methanol-water (0.1 mol/ml KH2PO4) (3:97), pH 3.7. The flow rate was 0.8 ml/min, fluorescence detection wave length lambdaex was 290 nm and lambdaem was 330 nm, column temperature was room temperature.


The detection limits were 0.02 nmol/ ml for NE, 0.01 nmol/ml for E, 0.04 nmol/ml for DA, and 0. 02 nmol/ml for 5-HT. RSD was in the range of 2.2% - 4.4%. The recoveries of all analytes were in the range of 82.9% - 93.6%. The linear range was 0.02 - 2.0 nmol/ml for NE, 0.01 - 3.3 nmol/ml for E, 0.04 - 3.6 nmol/ml for DA, and 0.02 - 1.6 nmol/ml for 5-HT. The related coefficient was in the range of 0.9977 - 0.9985.


The assay had been applied successfully to measure the contents of these four monoamines in blood plasma of the Alzheimer's patients.

[PubMed - indexed for MEDLINE]
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