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Bioresour Technol. 2013 Mar;132:432-5. doi: 10.1016/j.biortech.2013.01.061. Epub 2013 Feb 4.

Production of high-purity neofructooligosaccharides by culture of Xanthophyllomyces dendrorhous.

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  • 1Department of Bioengineering, Tatung University, 104 Taipei, Taiwan.


Neofructooligosaccharides (neo-FOS) were produced in submerged cultures of Xanthophyllomyces dendrorhous. Among the various strains of X. dendrorhous that have intracellular (6)G-fructofuranosidase ((6)G-FFase), BCRC 21346 with high enzyme activity (3.60 U/mL) and BCRC 22367 with low enzyme activity (0.59 U/mL) were investigated in this work. Neo-FOS were generated in a 5-L jar fermenter at 20°C, 100rpm and 2vvm with the pH controlled at 6.9±0.1, using 250g/L of sucrose as the substrate. Through the catalytic action of X. dendrorhous(6)G-FFase on sucrose, monosaccharides as well as neo-FOS were produced. A portion of these monosaccharides was consumed by the yeast cells. However, the production of monosaccharides was low in concentration in culture with low (6)G-FFase activity, indicating they might be used up concurrently during the fermentation. Consequently, neo-FOS at a purity of up to 87.4% could be obtained.

Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.

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