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J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Mar 15;921-922:9-14. doi: 10.1016/j.jchromb.2012.12.042. Epub 2013 Jan 24.

High throughput LC-MS/MS method for simultaneous determination of zidovudine, lamivudine and nevirapine in human plasma.

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  • 1Department of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, India; Axis Clinicals Limited, 1-121/1, Miyapur, Hyderabad 500 049, India.


A selective and sensitive high performance liquid chromatography-tandem mass spectrometry method has been developed and validated for simultaneous determination of zidovudine (ZDV), lamivudine (3TC) and nevirapine (NVP) in human plasma. After Solid phase extraction (SPE), analytes and ISTDs were run on Peerless Basic C18 column with an injection volume of 3μL and run time of 3.0min. An isocratic mobile phase of 0.1% formic acid in water:methanol (15:85, v/v) was used with positive mass spectrometric detection. The method was validated over a concentration range of 5-1500ng/mL for ZDV and 3TC and over the concentration range of 10-3000ng/mL for NVP. The intraday and interday precision and accuracy across four validation runs were ranged from 1.6 to 10.1% and 93.8 to 110.8% respectively.

Copyright © 2013 Elsevier B.V. All rights reserved.

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