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Biomed Opt Express. 2013 Feb 1;4(2):364-86. doi: 10.1364/BOE.4.000364. Epub 2013 Jan 30.

Imaging axonal transport in the rat visual pathway.

Author information

  • 1Discoveries in Sight Research Laboratories, Devers Eye Institute, and Legacy Research Institute, Legacy Health, Portland, OR 97232, USA.

Abstract

A technique was developed for assaying axonal transport in retinal ganglion cells using 2 µl injections of 1% cholera toxin b-subunit conjugated to AlexaFluor488 (CTB). In vivo retinal and post-mortem brain imaging by confocal scanning laser ophthalmoscopy and post-mortem microscopy were performed. The transport of CTB was sensitive to colchicine, which disrupts axonal microtubules. The bulk rates of transport were determined to be approximately 80-90 mm/day (anterograde) and 160 mm/day (retrograde). Results demonstrate that axonal transport of CTB can be monitored in vivo in the rodent anterior visual pathway, is dependent on intact microtubules, and occurs by active transport mechanisms.

KEYWORDS:

(170.2655) Functional monitoring and imaging; (170.3880) Medical and biological imaging

PMID:
23412846
[PubMed]
PMCID:
PMC3567722
Free PMC Article
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