Expression of caveolin-1 in the early phase of beta-TCP implanted in dog mandible

J Biomed Mater Res B Appl Biomater. 2013 Jul;101(5):804-12. doi: 10.1002/jbm.b.32884. Epub 2013 Feb 9.

Abstract

Caveolin is an essential and signature protein of caveolae. Caveolin-1 participates in signal transduction processes by acting as a scaffolding protein that concentrates, organizes and functional regulates signalling molecules within caveolar membranes. Beta-tricalcium phosphate (β-TCP) has been widely used for scaffold in tissue engineering due to its high biodegradability, osteoconductivity, easy manipulation, and lack of histotoxicity. To better understand the role of caveolin-1 in bone homeostasis and response to β-TCP scaffold, β-TCP was implanted into the dog mandible defects in beagle dogs, and gene expression profiles were examined focused on the molecular components involved in caveolin-1 regulation. Here we showed the quantitative imageology analysis characterized using in vivo micro-computed tomography (CT) images at 4 and 7 days after β-TCP implanted in dog mandibles. The bone reformation by using the β-TCP scaffolds began within 4 days of surgery, and was healing well at 7 days after surgery. Higher mRNA level of caveolin-1 was observed in β-TCP-implanted Beagle dog mandibles compared with controls at day 4 and day 7 post-surgery. The enhancement of caveolin-1 by β-TCP was further confirmed by immunohistochemistry and immunofluorescence analysis. We further revealed increased Smad7 and Phospho Stat3 expression in β-TCP-implanted specimens. Taken together, these results suggest that the enhancement of caveolin-1 play an important role in accelerating bone formation by β-TCP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Regeneration / drug effects
  • Bone Regeneration / genetics*
  • Bone Regeneration / physiology
  • Bone Substitutes / administration & dosage*
  • Calcium Phosphates / administration & dosage*
  • Caveolin 1 / genetics*
  • Caveolin 1 / metabolism
  • Dogs
  • Immunohistochemistry
  • Mandible / diagnostic imaging
  • Mandible / metabolism*
  • Mandible / surgery*
  • Materials Testing
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • STAT3 Transcription Factor / metabolism
  • Smad7 Protein / metabolism
  • Time Factors
  • Tissue Scaffolds / chemistry*
  • Transcriptome
  • X-Ray Microtomography

Substances

  • Bone Substitutes
  • Calcium Phosphates
  • Caveolin 1
  • RNA, Messenger
  • STAT3 Transcription Factor
  • Smad7 Protein
  • beta-tricalcium phosphate