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Anal Biochem. 2013 Jun 1;437(1):1-9. doi: 10.1016/j.ab.2013.01.030. Epub 2013 Feb 8.

Construction of an amperometric D-amino acid biosensor based on D-amino acid oxidase/carboxylated mutliwalled carbon nanotube/copper nanoparticles/polyalinine modified gold electrode.

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  • 1Department of Biochemistry, MD (Maharshi Dayanand) University, Rohtak, India.


An improved D-amino acid biosensor was constructed based on covalent immobilization of D-amino acid oxidase onto carboxylated mutliwalled carbon nanotube/copper nanoparticles/polyalinine hybrid film electrodeposited on gold electrode. The biosensor exhibited an optimal response within 2s at pH 8.0 and 30°C when polarized at 0.09 V. There was a linear relationship between biosensor response (μA) and D-alanine concentration ranging from 0.001 to 0.7 mM. The sensitivity of the biosensor was 54.85 μA cm(-2) mM(-1) with a lower limit of detection of 0.2 μM (signal/noise=3). The enzyme electrode was used 150 times over a period of 4 months. The biosensor measured the d-amino acid level in fruit juices.

Copyright © 2013 Elsevier Inc. All rights reserved.

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