Format

Send to:

Choose Destination
See comment in PubMed Commons below
Anticancer Res. 2013 Feb;33(2):393-9.

Protein kinases D2 and D3 are novel growth regulators in HCC1806 triple-negative breast cancer cells.

Author information

  • 1Department of Biochemistry, The University of Texas Health Science Center at Tyler, 11937 US Highway 271, Tyler, Texas 75708, USA.

Abstract

AIM:

The role of protein kinase D (PKD) in the context of breast cancer cell biology is not clear.

MATERIALS AND METHODS:

The expression of PKD isoforms was assessed in various breast cancer cell lines and PKD isoform-specific siRNAs and selective inhibitors were used to study the role of PKD in breast cancer cell growth.

RESULTS:

PKD2 and PKD3 were two major isoforms expressed at the highest levels in tumorgenic HCC1806 triple-negative breast cancer cells. Silencing PKD2 or PKD3 significantly inhibited HCC1806 cell proliferation, and PKD3 silencing had a higher inhibitory effect than PKD2 silencing on cell growth and PKD-mediated signaling. HCC1806 breast cancer cells were highly responsive to PKD inhibitors but not to a general protein kinase C (PKC) inhibitor.

CONCLUSION:

We have identified PKD2 and PKD3, especially PKD3, as novel cell growth regulators in HCC1806 triple-negative breast cancer cells. Targeting PKD instead of all PKCs effectively inhibited cell proliferation in a number of breast cancer cell lines.

PMID:
23393329
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk