Abstract
This study used multilocus sequence typing (MLST) of total DNA extracted from faecal specimens to genotype Helicobacter pylori to analyse intra-familial transmission. Faecal DNA was extracted and amplified by nested PCR. The products were analysed by direct sequencing and the allele type was determined using an MLST website. Mother-to-child transmission was suspected in at least two of three families, and father-to-child transmission was suspected in one family.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Antigens, Bacterial / analysis
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Child
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Child, Preschool
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DNA, Bacterial / genetics*
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DNA, Bacterial / isolation & purification
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Family
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Feces / chemistry
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Feces / microbiology*
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Female
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Helicobacter Infections / epidemiology
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Helicobacter Infections / microbiology*
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Helicobacter Infections / transmission*
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Helicobacter pylori / classification
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Helicobacter pylori / genetics*
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Humans
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Infant
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Infant, Newborn
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Japan / epidemiology
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Male
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Molecular Epidemiology
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Multilocus Sequence Typing / methods*
Substances
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Antigens, Bacterial
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DNA, Bacterial