Evaluation of B cell function in patients with HIV

Curr Protoc Immunol. 2013 Feb:Chapter 12:Unit 12.13.. doi: 10.1002/0471142735.im1213s100.

Abstract

HIV disease is associated with abnormalities in all major lymphocyte populations, including B cells. B cell dysfunction in HIV infection is largely driven by alterations in the subsets of B cells that circulate in the blood or reside in tissues. Meaningful functional assays are thus dependent on the identification and isolation of B cell subsets present in the starting material. This unit describes several assays designed to phenotype, fractionate, and assess functional properties of B cells that circulate in the blood of HIV-infected individuals. The four protocols, which have been adapted from standard techniques, are tailored to evaluate B cells in peripheral blood mononuclear cells (PBMCs) of individuals infected with HIV, but can also be applied to other disease settings and PBMCs isolated from healthy individuals.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • B-Lymphocytes / immunology*
  • Blood Circulation / immunology
  • Cell Culture Techniques / methods*
  • Cell Separation
  • HIV / immunology*
  • HIV Infections / diagnosis
  • HIV Infections / immunology*
  • Humans
  • Immunocompromised Host
  • Immunophenotyping / methods*
  • Leukocytes, Mononuclear / cytology
  • Lymphocyte Subsets / physiology*