Alpinate oxyphyllae fructus (Alpinia Oxyphylla Miq) extracts inhibit angiotensin-II induced cardiac apoptosis in H9c2 cardiomyoblast cells

Biosci Biotechnol Biochem. 2013;77(2):229-34. doi: 10.1271/bbb.120541. Epub 2013 Feb 7.

Abstract

Angiotensin II (Ang II) is a risk factor for cardiovascular disease. We used a traditional Chinese medicine, alpinate oxyphyllae fructus (AOF), to evaluate its effect on Ang II-induced cardiac apoptosis and mitochondrial dysfunction. Ang II-treated H9c2 cells were administered AOF of 20-100 µg/mL concentrations. Ang II significantly increased TUNEL-positive nuclei in the H9c2 cells, effect was inhibited by AOF administration in both pre-treated and post-treated H9c2 cells. Caspases 9 and 3 activities were increased by Ang II and downregulated by AOF administration, especially in pre-treatment. AOF treatment reversed Ang II-induced mitochondria membrane potential instability in H9c2 cells as observed by JC-1 stain assay. Furthermore, pro-apoptotic proteins Bad and cytochrome c increased and decreased respectively under AOF administration. The levels of p-Bad anti-apoptotic protein were significantly increased after AOF treatment. This study indicates that mitochondrial dependent apoptosis induced by Ang II.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alpinia / chemistry*
  • Angiotensin II / pharmacology*
  • Animals
  • Apoptosis / drug effects
  • Benzimidazoles
  • Carbocyanines
  • Cardiotonic Agents / isolation & purification
  • Cardiotonic Agents / pharmacology*
  • Caspase 3 / genetics
  • Caspase 3 / metabolism
  • Caspase 9 / genetics
  • Caspase 9 / metabolism
  • Cell Line
  • Cytochromes c / genetics
  • Cytochromes c / metabolism
  • Embryo, Mammalian
  • Fluorescent Dyes
  • Fruit / chemistry*
  • Gene Expression / drug effects
  • In Situ Nick-End Labeling
  • Membrane Potential, Mitochondrial / drug effects
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Myoblasts, Cardiac / cytology
  • Myoblasts, Cardiac / drug effects*
  • Myoblasts, Cardiac / metabolism
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Rats
  • bcl-Associated Death Protein / genetics
  • bcl-Associated Death Protein / metabolism

Substances

  • Bad protein, rat
  • Benzimidazoles
  • Carbocyanines
  • Cardiotonic Agents
  • Fluorescent Dyes
  • Plant Extracts
  • bcl-Associated Death Protein
  • Angiotensin II
  • 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine
  • Cytochromes c
  • Caspase 3
  • Caspase 9