Abstract
Angiotensin II (Ang II) is a risk factor for cardiovascular disease. We used a traditional Chinese medicine, alpinate oxyphyllae fructus (AOF), to evaluate its effect on Ang II-induced cardiac apoptosis and mitochondrial dysfunction. Ang II-treated H9c2 cells were administered AOF of 20-100 µg/mL concentrations. Ang II significantly increased TUNEL-positive nuclei in the H9c2 cells, effect was inhibited by AOF administration in both pre-treated and post-treated H9c2 cells. Caspases 9 and 3 activities were increased by Ang II and downregulated by AOF administration, especially in pre-treatment. AOF treatment reversed Ang II-induced mitochondria membrane potential instability in H9c2 cells as observed by JC-1 stain assay. Furthermore, pro-apoptotic proteins Bad and cytochrome c increased and decreased respectively under AOF administration. The levels of p-Bad anti-apoptotic protein were significantly increased after AOF treatment. This study indicates that mitochondrial dependent apoptosis induced by Ang II.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alpinia / chemistry*
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Angiotensin II / pharmacology*
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Animals
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Apoptosis / drug effects
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Benzimidazoles
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Carbocyanines
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Cardiotonic Agents / isolation & purification
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Cardiotonic Agents / pharmacology*
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Caspase 3 / genetics
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Caspase 3 / metabolism
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Caspase 9 / genetics
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Caspase 9 / metabolism
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Cell Line
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Cytochromes c / genetics
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Cytochromes c / metabolism
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Embryo, Mammalian
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Fluorescent Dyes
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Fruit / chemistry*
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Gene Expression / drug effects
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In Situ Nick-End Labeling
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Membrane Potential, Mitochondrial / drug effects
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Mitochondria / drug effects
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Mitochondria / metabolism
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Myoblasts, Cardiac / cytology
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Myoblasts, Cardiac / drug effects*
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Myoblasts, Cardiac / metabolism
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Rats
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bcl-Associated Death Protein / genetics
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bcl-Associated Death Protein / metabolism
Substances
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Bad protein, rat
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Benzimidazoles
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Carbocyanines
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Cardiotonic Agents
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Fluorescent Dyes
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Plant Extracts
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bcl-Associated Death Protein
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Angiotensin II
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5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine
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Cytochromes c
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Caspase 3
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Caspase 9