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J Vet Sci. 2013;14(1):61-7. Epub 2013 Feb 5.

Effects of conditioned media from human amniotic epithelial cells on corneal alkali injuries in rabbits.

Author information

  • 1Department of Veterinary Surgery and Ophthalmology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.

Abstract

This study was performed to evaluate the effects of conditioned media (CM) from human amniotic epithelial cells (HAECs) on the corneal wound healing process. Eighteen rabbits (36 eyes) were used and randomly assigned to three groups according treatment: CM from HAECs (group 1), vehicle alone (group 2), and saline (group 3). Corneal alkali injuries were induced with 1 N sodium hydroxide. Each reagent used for treatment evaluation was injected into the dorsal bulbar subconjunctiva and the area of the corneal epithelial defect was measured every other day. Two animals from each group were euthanized at a time on days 3, 7, and 15, and the cornea was removed for histological examination. The sum of the epithelial defect areas measured on day 0 to day 6 as well as day 0 to day 14 in group 1 was significantly smaller than those of other groups. Histological examination revealed that the group 1 corneas had less inflammatory cell infiltration and showed more intact epithelial features compared to the other groups. These results suggest that CM from HAECs promote corneal wound healing in rabbits.

KEYWORDS:

conditioned media; corneal alkali injury; human amniotic epithelial cell; rabbits

PMID:
23388445
[PubMed - indexed for MEDLINE]
PMCID:
PMC3615233
Free PMC Article
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