Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria

Mauricio J Lozano; María Eugenia Salas; María de los Angeles Giusti; María Carla Martini; José Luis López; Ileana Salto; María Florencia Del Papa; Mariano Pistorio; Antonio Lagares

doi:10.1016/j.mimet.2013.01.017

Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria

J Microbiol Methods. 2013 Apr;93(1):9-11. doi: 10.1016/j.mimet.2013.01.017.

Authors

Mauricio J Lozano¹, María Eugenia Salas, María de los Angeles Giusti, María Carla Martini, José Luis López, Ileana Salto, María Florencia Del Papa, Mariano Pistorio, Antonio Lagares

Affiliation

¹ IBBM-Instituto de Biotecnología y Biología Molecular, CCT-CONICET-La Plata, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata,Calles 47 y 115 (1900), La Plata, Argentina.

PMID: 23384825
DOI: 10.1016/j.mimet.2013.01.017

Abstract

The preparation of plasmid-borne RIVET libraries can be troublesome when high genomic coverages are needed. We present here the construction and functional validation of a new set of miniTn5 promoter traps to generate tnpR-based RIVET libraries. The ability to generate tnpR transcriptional fusions by transposition will significantly facilitate the setup of RIVET studies in those bacteria where Tn5 transposition is operative.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Transposable Elements
Gene Fusion
Gene Library
Genetics, Microbial / methods*
Gram-Negative Bacteria / genetics*
Molecular Biology / methods*
Promoter Regions, Genetic*

Substances

DNA Transposable Elements