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Nanomedicine (Lond). 2013 May;8(5):739-55. doi: 10.2217/nnm.12.151. Epub 2013 Feb 5.

Polyelectrolyte multilayer coating with two regulatory molecules on titanium: construction and its biological effects.

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  • 1Department of Oral & Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, 395 Yan'an Road, Hangzhou 310006, China.



This study aimed at constructing a novel disulfide-crosslinked collagen I/hyaluronic acid polyelectrolyte multilayer (PEM) coating incorporated with bFGF and arginine-glycine-aspartic acid on titanium via the layer-by-layer technique, and evaluating its biological effects.


The surface topography and components, thickness, degradation behaviors and bFGF release profiles of the PEM coating were investigated. The effects of the PEM coating on osteoprogenitor cell growth and bone implant interfacial binding strength in the rabbit femur model were evaluated separately.


The formation of disulfide bonds improved the stability of the PEM coating, resulting in a coating that can release bFGF in a slow and sustained manner. Biological evaluations revealed that the resultant PEM coating on titanium promoted various cell behaviors and enhanced the binding strength.


The employed cotreatment regimen enabled bFGF and arginine-glycine-aspartic acid to have a synergistic effect on the cell responses, which, in turn, improved the osseointegration.

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