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Nat Methods. 2013 Mar;10(3):239-42. doi: 10.1038/nmeth.2361. Epub 2013 Feb 3.

Synergistic and tunable human gene activation by combinations of synthetic transcription factors.

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  • 1Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.

Abstract

Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with combinations of engineered transcription activator-like effectors (TALEs). These combinations of TALE transcription factors induced substantial gene activation and allowed tuning of gene expression levels that will broadly enable synthetic biology, gene therapy and biotechnology.

PMID:
23377379
[PubMed - indexed for MEDLINE]
PMCID:
PMC3719416
Free PMC Article
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