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J Sep Sci. 2013 Mar;36(5):898-906. doi: 10.1002/jssc.201200831. Epub 2013 Feb 1.

Loading behavior of gatifloxacin in urine and lake water on a novel magnetic molecularly imprinted polymer used as extraction sorbent with spectrophotometric analysis.

Author information

  • 1Department of Analytical Chemistry, Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing, Jiangsu Province, China.

Abstract

The loading behavior of gatifloxacin (GTFX) in human urine and lake water on a novel magnetic molecularly imprinted polymer used as extraction sorbent with UV-Visible spectrometric analysis has been studied. The magnetic polymers had been prepared using GTFX as template molecule and Fe3O4 as magnetic component. The polymer had been characterized by SEM, Fourier-transform infrared spectrometry, and appropriate magnet separator. Parameters affecting the extraction efficiency were evaluated in order to achieve optimal loading and reduce nonspecific interactions. Good linearity of the method had been obtained in the range between 0.25 and 15 μg mL(-1) by UV-Vis spectrophotometry at 286 nm with spectral analysis from 240 to 400 nm. The method detection and quantification limits of GTFX in water were 0.075 and 0.25 μg mL(-1), respectively. This study showed good selectivity and loading efficiency (α > 2) of the polymers. The loading behavior of GTFX in the samples spiked on polymers had been obtained and each other with recovery higher than 91% with RSD% between 2.5 and 3.3. No pretreatment of samples were needed and no interference of compounds in urine and lake water were observed during adsorption.

© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PMID:
23371523
[PubMed - indexed for MEDLINE]
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