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Analyst. 2013 Mar 21;138(6):1645-8. doi: 10.1039/c3an36391k. Epub 2013 Jan 31.

Realization of on-tissue protein identification by highly efficient in situ digestion with graphene-immobilized trypsin for MALDI imaging analysis.

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  • 1Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, China.

Abstract

A novel implementation of in situ protein digestion supported by a graphene oxide-immobilized enzyme reactor (GO-IMER) in the MALDI imaging mass spectrometry (IMS) workflow is reported, which enables the simultaneous diagnostic identity and distribution attributes of the proteome on tissue.

PMID:
23364134
[PubMed - indexed for MEDLINE]
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