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Nat Biotechnol. 2013 Mar;31(3):227-9. doi: 10.1038/nbt.2501. Epub 2013 Jan 29.

Efficient genome editing in zebrafish using a CRISPR-Cas system.

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  • 1Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

Abstract

In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.

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PMID:
23360964
[PubMed - indexed for MEDLINE]
PMCID:
PMC3686313
Free PMC Article

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