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Appl Biochem Biotechnol. 2013 Mar;169(6):1811-21. doi: 10.1007/s12010-013-0108-3. Epub 2013 Jan 23.

Preparation, characterization, and bacteriostasis of AgNP-coated β-CD grafting cellulose beads.

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  • 1Heilongjiang Key Lab of Molecular Design and Preparation of Flame Retarded Materials, Department of Chemistry, Northeast Forestry University, Harbin, People's Republic of China. thundersking@yahoo.com.cn


A novel functional material of β-cyclodextrin (β-CD) grafting cellulose beads containing immobilized silver nanoparticles (AgNPs) is presented in this paper. The morphology was characterized by scanning electron microscopy, energy-dispersive X-ray, and X-ray photoelectron spectroscopy. Phenolphthalein probe molecule technique was used to detect the activity of the grafting β-CD, and the results demonstrated that the deposition of AgNPs had no influence on its encapsulation ability. Acid resistance of the AgNPs on the bead material was studied by atomic absorption spectrometry. The stability of the AgNPs was enhanced due to the grafting of β-CD. Tube dilution method was applied to study the bacteriostatic effect, and the minimal inhibitory doses of the novel material against Escherichia coli and Staphylococcus aureus were 12.5 and 25 mg, respectively. The minimal bactericidal doses for the two bacteria were 25 and 25 mg, respectively.

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