Display Settings:

Format

Send to:

Choose Destination
Korean J Radiol. 2013 Jan-Feb;14(1):45-50. doi: 10.3348/kjr.2013.14.1.45. Epub 2012 Dec 28.

Establishment of a protocol for determining gastrointestinal transit time in mice using barium and radiopaque markers.

Author information

  • 1Department of Radiology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Hwasun 519-763, Korea.

Abstract

OBJECTIVE:

The purpose of this study was to establish a minimally invasive and reproducible protocol for estimating the gastrointestinal (GI) transit time in mice using barium and radiopaque markers.

MATERIALS AND METHODS:

Twenty 5- to 6-week-old Balb/C female mice weighing 19-21 g were used. The animals were divided into three groups: two groups that received loperamide and a control group. The control group (n = 10) animals were administered physiological saline (1.5 mL/kg) orally. The loperamide group I (n = 10) and group II (n = 10) animals were administered 5 mg/kg and 10 mg/kg loperamide orally, respectively. Thirty minutes after receiving the saline or loperamide, the mice was administered 80 µL of barium solution and six iron balls (0.5 mm) via the mouth and the upper esophagus by gavage, respectively. Afterwards, the mice were continuously monitored with fluoroscopic imaging in order to evaluate the swallowing of the barium solution and markers. Serial fluoroscopic images were obtained at 5- or 10-min intervals until all markers had been excreted from the anal canal. For analysis, the GI transit times were subdivided into intestinal transit times (ITTs) and colon transit times (CTTs).

RESULTS:

The mean ITT was significantly longer in the loperamide groups than in the control group (p < 0.05). The mean ITT in loperamide group II (174.5 ± 32.3) was significantly longer than in loperamide group I (133.2 ± 24.2 minute) (p < 0.05). The mean CTT was significantly longer in loperamide group II than in the control group (p < 0.05). Also, no animal succumbed to death after the experimental procedure.

CONCLUSION:

The protocol for our study using radiopaque markers and barium is reproducible and minimally invasive in determining the GI transit time of the mouse model.

KEYWORDS:

Experimental study; Fluoroscopy; Gastrointestinal; Loperamide; Motility disorder

PMID:
23323030
[PubMed - indexed for MEDLINE]
PMCID:
PMC3542302
Free PMC Article

Images from this publication.See all images (3)Free text

Fig. 1
Fig. 2
Fig. 3
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for The Korean Radiological Society Icon for PubMed Central
    Loading ...
    Write to the Help Desk