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Appl Environ Microbiol. 2013 Mar;79(6):2096-8. doi: 10.1128/AEM.03112-12. Epub 2013 Jan 11.

Isolation of a substantial proportion of forest soil bacterial communities detected via pyrotag sequencing.

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  • 1Department of Microbiology & Immunology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada.

Abstract

We isolated 1,264 bacterial strains from forest soils previously surveyed via pyrosequencing of rRNA gene amplicons. Conventional culturing techniques recovered a substantial proportion of the community, with isolates representing 22% of 98,557 total pyrotags. Growth characteristics of isolates indicated that ecological traits were associated with relative abundances of corresponding pyrotag operational taxonomic units.

PMID:
23315727
[PubMed - indexed for MEDLINE]
PMCID:
PMC3592214
Free PMC Article

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