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Macromol Rapid Commun. 2013 Mar 12;34(5):399-405. doi: 10.1002/marc.201200711. Epub 2013 Jan 9.

A facile method for the preparation of monodisperse beads with uniform pore sizes for cell culture.

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  • 1Department of Biotechnology, The Catholic University of Korea, Gyeonggi-do, Republic of Korea.

Abstract

This paper describes a facile method for the preparation of porous gelatin beads with uniform pore sizes using a simple fluidic device and their application as supporting materials for cell culture. An aqueous gelatin droplet containing many uniform toluene droplets, produced in the fluidic device, is dropped into liquid nitrogen for instant freezing and the small toluene droplets evolve into pores in the gelatin beads after removal of toluene and then freeze-drying. The porous gelatin beads exhibit a uniform pore size and monodisperse diameter as well as large open pores at the surface. Fluorescence microscopy images of fibroblast-loaded gelatin beads confirm the attachment and proliferation of the cells throughout the porous gelatin beads.

Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PMID:
23303665
[PubMed - indexed for MEDLINE]
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