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PLoS One. 2012;7(12):e51361. doi: 10.1371/journal.pone.0051361. Epub 2012 Dec 27.

Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.

Author information

  • 1Beatson Institute for Cancer Research, University of Glasgow, Glasgow, United Kingdom.

Erratum in

  • PLoS One. 2013;8(5). doi:10.1371/annotation/4c610387-c7f0-4330-b772-30ed52a07547. Guitart, Amelie [corrected to Guitart, Amelie V].

Abstract

The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage.

PMID:
23300543
[PubMed - indexed for MEDLINE]
PMCID:
PMC3531453
Free PMC Article
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