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Luminescence. 2013 Nov-Dec;28(6):894-9. doi: 10.1002/bio.2453. Epub 2013 Jan 7.

Sensitive determination of DNA based on the interaction between prulifloxacin-terbium(III) complex and DNA.

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  • 1School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.

Abstract

A simple spectrofluorimetric method is described for the determination of DNA, based on its enhancement of the fluorescence intensity of prulifloxacin (PUFX)-Tb(3+). The luminescence intensity of the PUFX-Tb(3+) complex increased up to 10-fold after adding DNA. The excitation and emission wavelengths were 345 and 545 nm, respectively. Under optimum conditions, variations in the fluorescence intensity showed a good linear relationship with the concentration of hsDNA in the range of 3.0 × 10(-9) to 1.0 × 10(-6) g/mL, with a correlation coefficient (R) of 0.997, and the detection limit was 2.1 × 10(-9) g/mL. The method was successfully applied to the determination of DNA in synthetic samples, and recoveries were in the range 97.3-102.0%. The mechanism of fluorescence enhancement of the PUFX-Tb(3+) complex by DNA is also discussed. The mechanism may involve formation of a ternary complex mainly by intercalation binding together with weak electrostatic interaction, which will increase the energy transition from ligand to Tb(3+), increasing the rigidity of the complex, and decreasing the radiationless energy loss through O-H vibration of the H2O molecule in the PUFX-Tb(3+) complex. Compared with the previous DNA probes, the proposed method is not only more robust and friendly to the environment, but also of relatively higher sensitivity.

Copyright © 2013 John Wiley & Sons, Ltd.

KEYWORDS:

DNA; luminescence mechanism; prulifloxacin; spectrofluorimetry; terbium

PMID:
23297144
[PubMed - in process]
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