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Methods Mol Biol. 2013;951:45-55. doi: 10.1007/978-1-62703-146-2_4.

Use of boronic acid nanoparticles in glycoprotein enrichment.

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  • 1Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, China.

Abstract

Glyco-specific enrichment methods for mass spectrometry pretreatment are invaluable for the detection of low abundant glycoproteins or glycopeptides. For example, boronic acid can specifically interact with glycans in nonaqueous or basic aqueous solutions. Here, we describe a glyco-specific enrichment method which uses a boronic acid-functionalized "core-satellite" composite nanoparticle to isolate glycoproteins or glycopeptides from complex biological samples. Furthermore, we also demonstrate detection limit improvements and show how to evaluate the percent recovery from the glycoprotein or glycopeptide enrichment process via SDS-PAGE and (16)O/(18)O labeling strategies.

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