Display Settings:

Format

Send to:

Choose Destination
Cold Spring Harb Protoc. 2013 Jan 1;2013(1). pii: pdb.prot072769. doi: 10.1101/pdb.prot072769.

Investigating ion channel distribution using a combination of spatially limited photolysis, Ca(2+) imaging, and patch clamp recording.

Author information

  • 1Department of Pharmacology and Physiology, University of Rochester, Rochester, NY 14642, USA.

Abstract

The production of saliva by parotid acinar cells is stimulated by Ca(2+) activation of Cl(-) and K(+) channels located in the apical plasma membrane of these polarized cells. Here, we utilize a combination of spatially limited flash photolysis, Ca(2+) imaging, and electrophysiological recording to investigate the distinct distribution of Ca(2+)-dependent ion channels in the plasma membrane (PM) of enzymatically isolated murine parotid acinar cells. In these experiments, the aim of photolysis is to selectively target and modify the activity of ion channels, thereby revealing membrane-domain-specific differences in distribution. Specifically, the relative distribution of channels to either apical or basal PM can be investigated. Since there is substantial evidence that Ca(2+)-dependent Cl(-) channels are exclusively localized to the apical membrane of acinar cells, this provides an important electrophysiological verification that a particular membrane has been specifically targeted.

PMID:
23282644
[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk