Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Birth Defects Res A Clin Mol Teratol. 2013 Feb;97(2):69-78. doi: 10.1002/bdra.23105. Epub 2012 Dec 26.

An in vitro mouse model of congenital cytomegalovirus-induced pathogenesis of the inner ear cochlea.

Author information

  • 1Laboratory for Developmental Genetics, University of Southern California, Los Angeles, CA 90089, USA. mmelnick@usc.edu

Abstract

Congenital human cytomegalovirus (CMV) infection is the leading nongenetic etiology of sensorineural hearing loss (SNHL) at birth and prelingual SNHL not expressed at birth. The paucity of temporal bone autopsy specimens from infants with congenital CMV infection has hindered the critical correlation of histopathology with pathogenesis. Here, we present an in vitro embryonic mouse model of CMV-infected cochleas that mimics the human sites of viral infection and associated pathology. There is a striking dysplasia/hyperplasia in mouse CMV-infected cochlear epithelium and mesenchyme, including organ of Corti hair and supporting cells and stria vascularis. This is concomitant with significant dysregulation of p19, p21, p27, and Pcna gene expression, as well as proliferating cell nuclear antigen (PCNA) protein expression. Other pathologies similar to those arising from known deafness gene mutations include downregulation of KCNQ1 protein expression in the stria vascularis, as well as hypoplastic and dysmorphic melanocytes. Thus, this model provides a relevant and reliable platform within which the detailed cell and molecular biology of CMV-induced deafness may be studied.

Copyright © 2012 Wiley Periodicals, Inc.

PMID:
23281115
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for John Wiley & Sons, Inc.
    Loading ...
    Write to the Help Desk