Display Settings:

Format

Send to:

Choose Destination
Mycotoxin Res. 2013 May;29(2):79-88. doi: 10.1007/s12550-012-0155-6. Epub 2012 Dec 30.

Modified use of a commercial ELISA kit for deoxynivalenol determination in rice and corn silage.

Author information

  • 1Kobe Regional Center, Food and Agricultural Materials Inspection Center, 1-3-7 Minatojima-minami-machi, Kobe 650-0047, Japan.

Abstract

The analysis of deoxynivalenol (DON) in silage samples using enzyme-linked immunosorbent assay (ELISA) often leads to an overestimation. To better analyze DON in rice and corn silages using a commercially available ELISA kit, a cleanup method using a MultiSep #226 column was developed. As a result, overestimation of DON by the influence of specific cross-reaction with acetyldeoxynivalenol (AcDON) was confirmed. In samples where AcDON was not detected by liquid chromatography with mass spectrometry (LC-MS), no samples showed a significant difference (P < 0.05) in DON amounts between ELISA with cleanup and LC-MS analysis. For the recovery study, blank silage was spiked with 0.5 or 1.0 mg/kg DON. The mean recoveries of DON determined by ELISA with cleanup and LC-MS analysis were 112 and 96%, respectively, and the relative standard deviation for the repeatability (RSDr) were 8.2 and 9.8%, respectively. No samples showed a significant difference (P < 0.05) in DON concentration determined by either ELISA or LC-MS analysis. A collaborative study to validate this rapid method was carried out using four samples, two rice and two corn silage, by 10 participating laboratories. Each sample was analyzed using blind duplicates. The mean values of DON detected were 1.5-2.3 mg/kg, RSDr and the relative standard deviation for the reproducibility (RSDR) were 4.1-12.7 and 7.6-23.4%, respectively, and the HorRat values were 0.5-1.6. Therefore, the overestimation of DON by the influence of nonspecific cross-reaction with sample matrix was reduced by the cleanup method using a MultiSep #226 column, and analysis of DON in silage was improved. This use of this method for estimation of DON contamination in silage allows rapid detection at the place of use that is likely to result in improved animal health.

PMID:
23275133
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Springer
    Loading ...
    Write to the Help Desk