Interaction of cytochrome c with zinc oxide nanoparticles

Michaela Šimšíková; Marián Antalík

doi:10.1016/j.colsurfb.2012.10.058

Interaction of cytochrome c with zinc oxide nanoparticles

Colloids Surf B Biointerfaces. 2013 Mar 1:103:630-4. doi: 10.1016/j.colsurfb.2012.10.058. Epub 2012 Nov 26.

Authors

Michaela Šimšíková¹, Marián Antalík

Affiliation

¹ Department of Biochemistry, Faculty of Science, PJ Šafárik University, Šrobárova 2, 041 54 Košice, Slovakia.

PMID: 23274157
DOI: 10.1016/j.colsurfb.2012.10.058

Abstract

The influence of pH on the interaction between horse heart ferricytochrome c (cyt c) and zinc oxide nanoparticles (ZnO NPs) has been studied by a small angle scattering as well as UV-vis and FTIR spectroscopy. The observations showed that the optimal pH for the association of protein with nanoparticles is in pH range 5.0-8.0. Almost no significant change in structure and thermodynamic stability of cytochrome c after the association with 60 nm ZnO NPs was performed by UV-vis and by a circular dichroism spectroscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Animals
Circular Dichroism
Cytochromes c / metabolism*
Horses
Hydrogen-Ion Concentration
Microscopy, Atomic Force
Nanoparticles / chemistry*
Nanoparticles / ultrastructure
Particle Size
Protein Binding
Spectroscopy, Fourier Transform Infrared
Static Electricity
Temperature
Zinc Oxide / metabolism*

Substances

Cytochromes c
Zinc Oxide