Send to

Choose Destination
See comment in PubMed Commons below
Colloids Surf B Biointerfaces. 2013 Mar 1;103:624-9. doi: 10.1016/j.colsurfb.2012.08.039. Epub 2012 Sep 2.

Computational, electrochemical, and spectroscopic, studies of acetycholinesterase covalently attached to carbon nanotubes.

Author information

  • 1Instituto de Química de São Carlos, Universidade de São Paulo, CP 780, CEP: 13560-970, São Carlos, SP, Brazil.


This manuscript describes results related to the characterization of electrodes modified with a composite of acetylcholinesterase covalently bound to carbon nanotubes (CNT). The characterization was performed by computational methods and complemented by cyclic voltammetry, infrared spectroscopy, and electrochemical impedance spectroscopy. In-silico simulations enabled the identification of the binding site and the calculation of the interaction energy. Besides complementing the computational studies, experimental results obtained by cyclic voltammetry showed that the addition of CNT to the surface of electrodes yielded significant increases in effective area and greatly facilitated the electron transfer reactions. These results are also in agreement with impedance spectroscopy data, which indicated a high apparent rate constant, even after the immobilization of the enzyme. These results lend new information about the physical and chemical properties of biointerfaces at the molecular level, specifically about the mechanism and consequences of the interaction of a model enzyme with CNT.

Copyright © 2012 Elsevier B.V. All rights reserved.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk