Send to:

Choose Destination
See comment in PubMed Commons below
Meat Sci. 2013 Mar;93(3):463-8. doi: 10.1016/j.meatsci.2012.11.023. Epub 2012 Nov 16.

Prevalence and antimicrobial susceptibility of Escherichia coli O157:H7 on beef cattle slaughtered in Amman abattoir.

Author information

  • 1Department of Nutrition and Food Technology, Faculty of Agriculture, Jordan University of Science and Technology, Irbid, 22110, Jordan.


Cattle are the main asymptomatic reservoir of Escherichia coli O157:H7 which can cause illness to human. The objectives of the study were to measure the prevalence of E. coli O157:H7 on cattle slaughtered in Amman abattoir, detect virulence factors in the isolates, determine antibacterial resistance of the isolates, and know how the isolates are different or similar when compared to characterized isolates from developed countries. A total of 540 samples (feces, hide, and carcass) were tested for E. coli O157:H7 using the method of ISO 16654:(E). Conventional and multiplex PCR assays were used for serotype confirmation and virulence factor detection, respectively. Fifty E. coli O157:H7 isolates were identified and virulence factors eaeA and hlyA were present in all of the isolates. 60%, 12%, and 22% of the isolates harbored stx(1), stx(2), and stx(1) and stx(2), respectively. The prevalence rates of enterotoxigenic E. coli O157:H7 (n=47) were 8.3%, 10%, and 7.8% in feces, hides and carcasses, respectively. The antimicrobial profiles of the isolates showed an extensive resistance to erythromycin, neomycin and vancomycin and high sensitivity to ampicillin, ciprofloxacin, gentamicin, kanamycin and tetracycline.

Copyright © 2012 Elsevier Ltd. All rights reserved.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk