Chryseomicrobium amylolyticum sp. nov., isolated from a semi-arid tropical soil, and emended descriptions of the genus Chryseomicrobium and Chryseomicrobium imtechense

Int J Syst Evol Microbiol. 2013 Jul;63(Pt 7):2612-2617. doi: 10.1099/ijs.0.044552-0. Epub 2012 Dec 21.

Abstract

A rod-shaped, non-motile, Gram-stain-positive, catalase-positive, starch-hydrolysing strain, JC16(T), was isolated from a semi-arid tropical soil from India. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain JC16(T) clustered with the type species of the genus Chryseomicrobium, Chryseomicrobium imtechense MW 10(T), a member of the family Planococcaceae within the phylum Firmicutes with 99.3 % sequence similarity. Major (>10 %) fatty acids of strain JC16(T) were iso-C15 : 0 and iso-C16 : 0. Minor (<10 and >1 %) amounts of C16 : 0, iso-C14 : 0, anteiso-C15 : 0, iso-C17 : 0, anteiso-C17 : 0, iso-C17 : 1ω10c and C16 : 1ω11c are present in strain JC16(T). Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids (PL2-4), aminolipids (AL1, 2) and an unknown lipid. Cell wall peptidoglycan was of the type l-Orn-D-Glu. The quinone system was composed of MK-7, MK-8 and MK-6. Genomic DNA G+C content of strain JC16(T) was 57.6 mol%. Distinct physiological, chemotaxonomic and genotypic differences (37 % reassociation based on DNA-DNA hybridization) from Chryseomicrobium imtechense MW 10(T) support the classification of strain JC16(T) as a representative of a novel species in the genus Chryseomicobium, for which the name Chryseomicrobium amylolyticum sp. nov. (type strain JC16(T) = DSM 23442(T) = NBRC 105215(T)) is proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • India
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phospholipids / analysis
  • Phylogeny*
  • Planococcaceae / classification*
  • Planococcaceae / genetics
  • Planococcaceae / isolation & purification
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Tropical Climate
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / analysis

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 6

Associated data

  • GENBANK/FM209428