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Cell Div. 2012 Dec 20;7(1):25. doi: 10.1186/1747-1028-7-25.

Mitofusin 1 is degraded at G2/M phase through ubiquitylation by MARCH5.

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  • 1Department of Biochemistry, Ajou University School of Medicine, Suwon, South Korea. hscho@ajou.ac.kr.

Abstract

BACKGROUND:

Mitochondria exhibit a dynamic morphology in cells and their biogenesis and function are integrated with the nuclear cell cycle. In mitotic cells, the filamentous network structure of mitochondria takes on a fragmented form. To date, however, whether mitochondrial fusion activity is regulated in mitosis has yet to be elucidated.

FINDINGS:

Here, we report that mitochondria were found to be fragmented in G2 phase prior to mitotic entry. Mitofusin 1 (Mfn1), a mitochondrial fusion protein, interacted with cyclin B1, and their interactions became stronger in G2/M phase. In addition, MARCH5, a mitochondrial E3 ubiquitin ligase, reduced Mfn1 levels and the MARCH5-mediated Mfn1 ubiquitylation were enhanced in G2/M phase.

CONCLUSIONS:

Mfn1 is degraded through the MARCH5-mediated ubiquitylation in G2/M phase and the cell cycle-dependent degradation of Mfn1 could be facilitated by interaction with cyclin B1/Cdk1 complexes.

PMID:
23253261
[PubMed]
PMCID:
PMC3542011
Free PMC Article
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