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Clin Dev Immunol. 2012;2012:478052. doi: 10.1155/2012/478052. Epub 2012 Nov 1.

The average IFN-γ secreting capacity of specific CD8(+) T cells is compromised while increasing copies of a single T cell epitope encoded by DNA vaccine.

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  • 1Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.

Abstract

Previous studies suggested that both the frequency and the mean fluorescence intensity (MFI) of cytokine secreting T cells could be of great value for immunogenicity evaluation of a vaccine. In this study, by constructing epitope-based DNA vaccines encoding a previously identified CD8(+) T cell epitope, we investigated the influence of multiplying epitope copies on both the frequency and the MFI of specific IFN-γ secreting CD8(+) T cells. We found that frequencies of specific CD8(+) T cell could be improved by multiplying epitope copies, while the MFI of IFN-γ secreted by epitope-specific CD8(+) T cells decreased synchronously. And further analysis showed that the decrease of MFI was not caused by the functional avidity variation of CD8(+) T cell receptor.

PMID:
23251217
[PubMed - indexed for MEDLINE]
PMCID:
PMC3509377
Free PMC Article

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