Format

Send to:

Choose Destination
See comment in PubMed Commons below
Am J Pathol. 2013 Feb;182(2):332-8. doi: 10.1016/j.ajpath.2012.11.002. Epub 2012 Dec 13.

Light microscopic visualization of podocyte ultrastructure demonstrates oscillating glomerular contractions.

Author information

  • 1Department II of Internal Medicine and the Center for Molecular Medicine Cologne, University of Cologne, 50937 Cologne, Germany.

Abstract

Podocytes, the visceral epithelial cells of the kidney glomerulus, elaborate primary and interdigitating secondary extensions to enwrap the glomerular capillaries. A hallmark of podocyte injury is the loss of unique ultrastructure and simplification of the cell shape, called foot process effacement, which is a classic feature of proteinuric kidney disease. Although several key pathways have been identified that control cytoskeletal regulation, actin dynamics, and polarity signaling, studies into the dynamic regulation of the podocyte structure have been hampered by the fact that ultrastructural analyses require electron microscopic imaging of fixed tissue. We developed a new technique that allows for visualization of podocyte foot processes using confocal laser scanning microscopy. The combination of inducible and mosaic expression of membrane-tagged fluorescent proteins in a small subset of podocytes enabled us to acquire light microscopic images of podocyte foot processes in unprecedented detail, even in living podocytes of freshly isolated glomeruli. Moreover, this technique visualized oscillatory glomerular contractions and confirmed the morphometric evaluations obtained in static electron microscopic images of podocyte processes. These data suggest that the new technique will provide an extremely powerful tool for studying the dynamics of podocyte ultrastructure.

Copyright © 2013 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

PMID:
23246153
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk