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Zhong Yao Cai. 2012 Jun;35(6):863-6.

[Apoptotic mechanism of gecko crude peptides on human liver carcinoma cell line HepG2].

[Article in Chinese]

Author information

  • 1Medical School and the Key Laboratory of Pharmacology and Medical Molecular Biology, Henan University of Science and Technology, Luoyang 471003, China. SYing2009@126.com

Abstract

OBJECTIVE:

To investigate the effect of Gecko crude peptides (GCPS) on human liver carcinoma HepG2 cells and its mechanism.

METHODS:

MTT assay was used to analyze the effect of the GCPS on the proliferation of HepG2 Cell; Nucleus change of HepG2 treated with GCP was observed by Hoechst33258 fluorescence staining, and BAX and BCL-2 were detected with western-blot assay.

RESULTS:

GCPS could inhibit the proliferation of HepG2 Cell in a time and dosage dependent way, and its half-maximal inhibitory concentration (IC50) was 1.2 mg/mL; HepG2 pretreated with GCPS showed apoptotic morphological changes. GCPS (1.6 mg/mL, 0.8 mg/mL) could decrease the expression of BCL-2 protein, and increase the expression of BAX protein.

CONCLUSION:

GCPS can inhibit the proliferation of HepG2 cell. The mechanism may be related to the induction apoptosis of HepG2.

PMID:
23236815
[PubMed - indexed for MEDLINE]
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