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Zhong Yao Cai. 2012 Jun;35(6):863-6.

[Apoptotic mechanism of gecko crude peptides on human liver carcinoma cell line HepG2].

[Article in Chinese]

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  • 1Medical School and the Key Laboratory of Pharmacology and Medical Molecular Biology, Henan University of Science and Technology, Luoyang 471003, China.



To investigate the effect of Gecko crude peptides (GCPS) on human liver carcinoma HepG2 cells and its mechanism.


MTT assay was used to analyze the effect of the GCPS on the proliferation of HepG2 Cell; Nucleus change of HepG2 treated with GCP was observed by Hoechst33258 fluorescence staining, and BAX and BCL-2 were detected with western-blot assay.


GCPS could inhibit the proliferation of HepG2 Cell in a time and dosage dependent way, and its half-maximal inhibitory concentration (IC50) was 1.2 mg/mL; HepG2 pretreated with GCPS showed apoptotic morphological changes. GCPS (1.6 mg/mL, 0.8 mg/mL) could decrease the expression of BCL-2 protein, and increase the expression of BAX protein.


GCPS can inhibit the proliferation of HepG2 cell. The mechanism may be related to the induction apoptosis of HepG2.

[PubMed - indexed for MEDLINE]
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