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Zhongguo Zhong Yao Za Zhi. 2012 Sep;37(17):2618-21.

[Anti-inflammatory effect and mechanism of artemisinin and dihydroartemisinin].

[Article in Chinese]

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  • 1School of Pharmacy, Yantai University, Yantai 264005, China.



To study and compare the anti-inflammatory effect and molecular mechanism of artemisinin and dihydroartemisinin.


Mouse mononuclear macrophage RAW264.7 cells were stimulated to release inflammatory mediators such as TNF-alpha, IL-6 and NO, in order to assess the drugs' inhibitory effect on macrophage's release of above inflammatory mediators. The levels of TNF-alpha and IL-6 were determined by ELISA and the cytotoxicity was determined by MTT method. The protein expression of iNOS, COX-2 and beta-actin were tested by Western blot. The enzymatic activity of COX-2 was determined by colorimetric method.


Dihydroartemisinin significantly inhibited LPS-induced release of TNF-alpha, IL-6 and NO from RAW264.7 in mice with the concentration range of 12.5 - 100 micromol x L(-1), and showed good dose dependence. Artemisinin only inhibited the IL-6 release to a certain extent.


Dihydroartemisinin inhibits macrophages from releasing inflammatory factors TNF-alpha and IL-6 and inflammatory mediators NO by down-regulating iNOS protein. Artemisinin may help dihydroartemisinin to show its anti-inflammatory effect through metabolism.

[PubMed - indexed for MEDLINE]
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