Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Dec;28(12):1261-4.

[Isolation and characterization of human breast tumor stem cells].

[Article in Chinese]

Author information

  • 1Institute of Stem Cells, General Hospital, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China. wanglinbin007@126.com



To isolate and culture human breast cancer stem cells, and characterize their biological properties in vitro.


Fresh tumor tissues of breast cancer patients were collected from surgical rooms. The tissue samples were mechanically sheared and enzyme-digested to get single cell suspensions. Cancer cell surface antigens were analyzed by flow cytometry, and cell growth curve was established by MTT assay; Real-time quantitative PCR (qPCR) was employed to detect the expressions of stem cell-specific genes Sox2 and Nanog in the cancer cells; Immunofluorescence staining was performed to examine the expressions of breast cancer-specific proteins Bcl-2 and progesterone receptor (PR).


Human breast cancer stem cells grew in the form of spheres. These stem cells had a CD44(+);/CD24(-); phenotype as characterized by flow cytometry, expressed high levels of Sox2 and Nanog genes, and were positive for Bcl-2 and PR.


Human breast tumors contain CD44(+);/CD24(-); cancer stem cells which are capable of self-renewal and proliferation, and can be long-term cultured and expanded in vitro.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk