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Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Dec;28(12):1261-4.

[Isolation and characterization of human breast tumor stem cells].

[Article in Chinese]

Author information

  • 1Institute of Stem Cells, General Hospital, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China. wanglinbin007@126.com

Abstract

AIM:

To isolate and culture human breast cancer stem cells, and characterize their biological properties in vitro.

METHODS:

Fresh tumor tissues of breast cancer patients were collected from surgical rooms. The tissue samples were mechanically sheared and enzyme-digested to get single cell suspensions. Cancer cell surface antigens were analyzed by flow cytometry, and cell growth curve was established by MTT assay; Real-time quantitative PCR (qPCR) was employed to detect the expressions of stem cell-specific genes Sox2 and Nanog in the cancer cells; Immunofluorescence staining was performed to examine the expressions of breast cancer-specific proteins Bcl-2 and progesterone receptor (PR).

RESULTS:

Human breast cancer stem cells grew in the form of spheres. These stem cells had a CD44(+);/CD24(-); phenotype as characterized by flow cytometry, expressed high levels of Sox2 and Nanog genes, and were positive for Bcl-2 and PR.

CONCLUSION:

Human breast tumors contain CD44(+);/CD24(-); cancer stem cells which are capable of self-renewal and proliferation, and can be long-term cultured and expanded in vitro.

PMID:
23232517
[PubMed - indexed for MEDLINE]
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