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Immun Ageing. 2012 Dec 11;9(1):27. doi: 10.1186/1742-4933-9-27.

Defective pro-IL-1β responses in macrophages from aged mice.

Author information

  • 1Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, S6-862, 55 Lake Avenue North, Worcester, MA, 01655, USA. anuja.mathew@umassmed.edu.

Abstract

BACKGROUND:

Cytokines regulated by the inflammasome pathway have been extensively implicated in various age-related immune pathologies. We set out to elucidate the contribution of the nod-like receptor protein 3 (NLRP3) inflammasome pathway to the previously described deficiencies in IL-1β production by macrophages from aged mice. We examined the production of pro-IL-1β and its conversion into IL-1β as two separate steps and compared these cytokine responses in bone marrow derived macrophages from young (6-8 weeks) and aged (18-24 months) C57BL/6 mice.

FINDINGS:

Relative to macrophages from young mice, macrophages from aged mice produced less pro-IL-1β after TLR4 stimulation with LPS. However upon activation of the NLRP3 inflammasome with ATP, macrophages from young and aged mice were able to efficiently convert and secrete intracellular pro-cytokines as functional cytokines.

CONCLUSIONS:

Lower levels of IL-1β production are a result of slower and lower overall production of pro-IL-1β in macrophages from aged mice.

PMID:
23228123
[PubMed]
PMCID:
PMC3545921
Free PMC Article
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