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J Microbiol Methods. 2013 Feb 15;92(2):197-200. doi: 10.1016/j.mimet.2012.11.020. Epub 2012 Dec 5.

Rapid and sensitive detection of Acinetobacter baumannii using loop-mediated isothermal amplification.

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  • 1Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan.

Abstract

Here we report the design and evaluation of a loop-mediated isothermal amplification (LAMP) assay for detecting Acinetobacter baumannii DNA based on the 16S-23S rRNA intergenic spacer (ITS) sequence. The results showed that target DNA was amplified and visualized within 30min and with a detection limit 100-fold greater than PCR.

Copyright © 2013 Elsevier B.V. All rights reserved.

PMID:
23220188
[PubMed - indexed for MEDLINE]
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