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Anal Biochem. 2013 Mar 1;434(1):181-6. doi: 10.1016/j.ab.2012.11.022. Epub 2012 Dec 5.

In vivo detection and quantification of chemicals that enhance protein stability.

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  • 1Howard Hughes Medical Institute, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.


We have devised protein-folding sensors that link protein stability to TEM-1 β-lactamase activity. The addition of osmolytes and other compounds with chemical chaperone activity to the growth medium of bacteria containing these sensors increases β-lactamase activity up to 207-fold in a dose-dependent manner. This enables the rapid detection and sensitive quantification of compounds that enhance in vivo protein stability.

Copyright © 2012 Elsevier Inc. All rights reserved.

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