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Zhong Yao Cai. 2012 May;35(5):675-8.

[Callus cultivation and determination of gentiopicroside from Gentiana macrophylla].

[Article in Chinese]

Author information

  • 1Department of Biology, Gansu College of TCM, Lanzhou 730000, China. swjys@gszy.edu.cn

Abstract

OBJECTIVE:

To explore and improve the utilization efficiency of Gentiana macrophylla resources.

METHODS:

The germination of Gentiona macrophylla seeds were used as explants to induce calluses; Cultured on various culture media and condition for callus induction and subculture by test; The content of gentiopicroside in callus was determined by HPLC.

RESULTS:

The optimum medium for callus was MS +2,4-D (1.0 mg/L) + 6-BA (0.5 mg/L), and MS +2,4-D (1.0 mg/L) +6-BA (0.5 mg/L) + NAA (0.5 mg/L) was the optimum for subculture,the optimum time of subculture was 30 d; The accumulated gentiopicroside reached the highest level with 0.78 mg/g DW when entered 28-30 d.

CONCLUSION:

The optimized culture method of callus of Gentiana. macrophylla is obtained; The growth curve of callus of Gentiona macrophylla turns on "S" appearance and the accumulated gentiopicroside increases along with the callus appreciation.

PMID:
23213725
[PubMed - indexed for MEDLINE]

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