The transmissibility of trypanosome infection to N'Dama cattle by tsetse flies caught in the field was examined. Wild-caught Glossina palpalis gambiensis were transferred singly into small numbered cages and allowed to feed on 14 uninfected N'Dama cattle. Following a completed feed the tsetse were dissected and infection in the proboscis, the salivary glands and the gut was recorded. Each animal was bitten by a number of tsetse ranging from five up to 64 flies. Following dissection of the tsetse flies, seven of the cattle were found to have been bitten by a single infected tsetse, five by two, while the remaining two were each fed upon by three infected tsetse. The tsetse were harbouring either Trypanosoma vivax or a trypanosome species belonging to the Nannomonas subgenus or both species. The experimental animals were monitored daily over a period of three months for the appearance of trypanosomes in the blood and for antibodies in their sera. Other parameters such as body temperature, local skin reactions, packed red cell volume and weight changes were also measured. Trypanosomes were first detected eight days after the infective bite. Only five of the 14 cattle became infected, of which three had been exposed to a single infected tsetse fly. Trypanosoma vivax was detected in one animal, Trypanosoma congolense in two cattle and mixed infections of both species in the remaining two animals. These findings show that N'Dama cattle can become infected with trypanosomes through the bite of a single infected tsetse fly under field conditions. However, only five (possibly eight) of the 23 infected tsetse were able to transmit the parasites successfully.