Format

Send to:

Choose Destination
See comment in PubMed Commons below
Colloids Surf B Biointerfaces. 2013 Mar 1;103:231-7. doi: 10.1016/j.colsurfb.2012.10.005. Epub 2012 Oct 24.

Nanostructured biosensors built with layer-by-layer electrostatic assembly of hemoglobin and Fe₃O₄@Pt nanoparticles.

Author information

  • 1Institute of Analytical Chemistry for Life Science, School of Public Health, Nantong University, Nantong 226019, PR China.

Abstract

This paper reports layer-by-layer (LBL) films fabricated with hemoglobin and core-shell nanoparticles with Fe(3)O(4) as the core covered by Pt (Fe(3)O(4)@Pt) and their applications in biosensing. The characterization of {Hb/Fe(3)O(4)@Pt}(n) LBL films at different layers revealed that the formation of films is step-by-step and uniform. Meanwhile, at glassy carbon electrodes modified with {Hb/Fe(3)O(4)@Pt}(n) film at different layers there was a pair of well-defined and nearly reversible peaks in cyclic voltammetry (CV). CV results indicated that the electroactivity of the structure with four bilayers was the best. The {Hb/Fe(3)O(4)@Pt}(4) film modified electrode could be used to detect H(2)O(2) and nitrite with the linear range from 0.125 μM to 0.16 mM for H(2)O(2) and 1.5 μM to 0.12 mM for nitrite as well as the detection limits of 0.03 μM for H(2)O(2) and 0.29 μM for nitrite (S/N=3). The biosensors also exhibited good reproducibility, high selectivity, and long-term stability. Our investigation showed that the strategy taking advantages of Fe(3)O(4)@Pt and LBL assembly is ideal for direct electrochemistry of redox proteins as well as the sensitive and stable mediator-free biosensors.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID:
23201742
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk