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J Hosp Infect. 2013 Feb;83(2):164-6. doi: 10.1016/j.jhin.2012.10.006. Epub 2012 Nov 30.

Comparison of two selective media for the recovery of Clostridium difficile from environmental surfaces.

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  • 1Microbiology Department, Freeman Hospital, Newcastle upon Tyne, UK. kerry.hill@nuth.nhs.uk


Two culture media were compared for their ability to isolate Clostridium difficile from environmental sites within a UK hospital. The media were cefoxitin-cycloserine-egg yolk agar plus lysozyme (CCEY/L) and chromID C. difficile. A wide range of environmental surfaces was sampled using sterile sponges (Polywipes) and these were inoculated on to both media. C. difficile was recovered from 105 of 496 sites (21%) using a combination of both media. The sensitivity of chromID C. difficile was 87.6% compared with 26.6% for CCEY/L (P < 0.0001). chromID C. difficile performed significantly better than CCEY/L for the recovery of C. difficile from the environment.

Copyright © 2012 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

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