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FEBS Lett. 2013 Jan 4;587(1):94-7. doi: 10.1016/j.febslet.2012.11.017. Epub 2012 Nov 27.

Spectroscopic characterization of the acid-alkaline transition of a thermophilic cytochrome P450.

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  • 1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.

Abstract

The spectroscopic properties of thermophilic cytochrome P450 from the thermoacidophilic crenarchaeon Sulfolobus tokodaii strain 7 (P450st) were investigated in acidic and basic solutions. The resting form of ferric-P450st in weakly-acidic and neutral solutions contained a thiolate/H₂O coordinated low-spin heme. Below pH 1.5, P450st underwent cleavage of the Fe-S bond and was converted into apo-P450st. Above pH 8, the acid-alkaline transition due to the deprotonation of the water ligand was observed. The produced thiolate/OH⁻-coordinated ferric-P450st was stable at room temperature. The pK(a) value of 8.7 for the transition reflects the protonation properties of the distal side of the heme.

Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

PMID:
23195688
[PubMed - indexed for MEDLINE]
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