Efficient cell reprogramming using bioengineered surfaces

Adv Healthc Mater. 2012 Mar;1(2):177-82. doi: 10.1002/adhm.201200017. Epub 2012 Feb 16.

Abstract

A novel method for cell reprogramming is been developed by immobilizing nucleic acid transfer vectors containing free amino groups, like lentiviral particles, onto pentafluorophenyl methacrylate (PFM)-modified surfaces obtained by PFM grafting affter Ar plasma treatment. This technique is able to reprogram murine somatic cells into pluripotent cells at high efficiencies. We call these modified surfaces cell reprogramming surfaces, or CRS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry*
  • Cell Line
  • DNA / chemistry*
  • DNA / genetics*
  • Materials Testing
  • Mice
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / physiology*
  • Surface Properties
  • Transfection / methods*

Substances

  • Biocompatible Materials
  • DNA