FEBS Lett. 1990 Mar 12;262(1):104-6.

The ribonuclease from an extinct bovid ruminant.

Stackhouse J, Presnell SR, McGeehan GM, Nambiar KP, Benner SA.

Source

Laboratory for Organic Chemistry, E.T.H., Zurich, Switzerland.

Abstract

The sequence of the ribonuclease from the ancestor of swamp buffalo, river buffalo, and ox, corresponding approximately to Pachyportax latidens, an extinct ruminant known from the fossil record, has been reconstructed using the rule of 'maximum parsimony'. This protein and two sequences that may have been intermediates in the evolution of modern ribonuclease have been constructed in the laboratory by site-directed mutagenesis, and their properties examined.

PMID:: 2318301; [PubMed - indexed for MEDLINE]

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