Periradicular surgery aims to achieve complete wound healing and regeneration of the periodontal unit. Platelet-rich fibrin (PRF) is a platelet concentrate that is widely used to promote tissue healing and bone induction through its various growth factors and has been used in this study. This case report describes the use of PRF unilaterally in a bilateral periapical lesion in the same patient. The site treated with PRF showed better healing than the other site. This was confirmed with a 3-D X-ray.