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Int J Ophthalmol. 2012;5(5):555-61. doi: 10.3980/j.issn.2222-3959.2012.05.03. Epub 2012 Oct 18.

Homemade lyophilized cross linking amniotic sustained-release drug membrane with anti-scarring role after filtering surgery in rabbit eyes.

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  • 1Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.

Abstract

AIM:

To investigate the antifibrotic effect of the freeze-dried bilayered fibrin-binding amniotic membrane as a drug delivery system on glaucoma surgery in rabbit model. The aim of this study was to prepare a novel local delivery system for the sustained and controllable release of 5-Fu.

METHODS:

Twenty-four Japanese white rabbits were randomized into three groups: the experimental group (ocular trabeculectomy in combination with 5-Fu loaded freeze-dried bilayered fibrin-binding amniotic membrane transplantation), the control group (ocular trabeculectomy in combination with 5-Fu) and the blank group (single trabeculectomy). HE staining, massion staining and immunohistochemistry for α-SMA were performed on days 7, 14, 21 and 30 following surgery. The concentration of 5-Fu in rabbit aqueous humor was examined by high performance liquid chromatography (HPLC) 3 days after the surgery.

RESULTS:

Statistical differences were noted in intraocular pressure among groups on day 7, 14, 21 and 30 following surgery. Histology further demonstrated that trabeculectomy in combination with freeze-dried bilayered fibrin-binding amniotic membrane yielded well wound healing and no scar formation and was beneficial for long term effect.

CONCLUSION:

HPLC showed a good slow-release effect with freeze-dried bilayered fibrin-binding amniotic membrane.

KEYWORDS:

drug delivery system; filtering surgery; freeze-dried bilayered fibrin-binding amniotic membrane; glaucoma; inhibition of scarring formation

PMID:
23166864
[PubMed]
PMCID:
PMC3484711
Free PMC Article
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