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Methods Mol Biol. 2013;962:227-36. doi: 10.1007/978-1-62703-236-0_19.

ChIP sequencing to identify p53 targets.

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  • 1Department of Integrative Life Sciences, Virginia Commonwealth University, Richmond, VA, USA.

Abstract

Mutant p53 may activate target genes through the interaction of transcription factors or through histone modifications. Chromatin immunoprecipitation (ChIP) is a method commonly used to study these types of protein interactions. In order to generate a list of target genes that may be activated through this mechanism, ChIP sequencing may be used. ChIP sequencing involves the mass parallel sequencing of ChIP DNA fragments. We describe a method by which to prepare chromatin immunoprecipitation sequencing libraries and how to analyze sequencing data. In this procedure, prepared libraries have been sent to a core facility. The results have been verified using quantitative PCR.

[PubMed - indexed for MEDLINE]
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